Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells-Reference-Cited by-同舟云学术

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Published:2020-12-01 Issue:4 Volume:8 Page:176-182
ISSN:2544-3577
Container-title:Medical Journal of Cell Biology
language:en
Short-container-title:

Author:

Kocherova Ievgeniia¹,Stefańska Katarzyna²,Bryl Rut¹,Perek Joanna¹,Pieńkowski Wojciech³,Zakova Jana⁴,Crha Igor⁴⁵,Ventruba Pavel⁴,Mozdziak Paul⁶,Ješeta Michal⁴⁷

Affiliation:

1. Department of Anatomy, Poznań University of Medical Sciences , Poznań , Poland

2. Department of Histology and Embryology, Poznań University of Medical Sciences , Poznań , Poland

3. Division of Perinatology and Women’s Diseases, Poznań University of Medical Sciences , Poznań , Poland

4. Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno , Brno , Czech Republic

5. Department of Nursing and Midwifery, Faculty of Medicine, Masaryk University , Brno , Czech Republic

6. Physiology Graduate Program, North Carolina State University , Raleigh, NC , USA

7. Department of Veterinary Sciences, Czech University of Life Sciences in Prague , Prague , Czech Republic

Abstract

Abstract Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points. Running title: Apoptosis-related genes expression in granulosa cells in vitro

Publisher

Walter de Gruyter GmbH

Subject

Cell Biology,Molecular Biology

Link

https://www.sciendo.com/pdf/10.2478/acb-2020-0023

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