Involvement of Aberrant DNA Methylation in the Deregulated Expression of EHF, LPAR1, MPZL3, and POPDC2 Genes in Equine Sarcoids-Reference-Cited by-同舟云学术

Involvement of Aberrant DNA Methylation in the Deregulated Expression of EHF, LPAR1, MPZL3, and POPDC2 Genes in Equine Sarcoids

Published:2024-01-01 Issue:1 Volume:24 Page:99-107
ISSN:2300-8733
Container-title:Annals of Animal Science
language:en
Short-container-title:

Author:

Semik-Gurgul Ewelina¹,Gurgul Artur²,Pawlina-Tyszko Klaudia¹,Gałuszka Anna³,Pędziwiatr Rafał⁴⁵,Witkowski Maciej⁴⁶,Ząbek Tomasz¹

Affiliation:

1. 1 Department of Animal Molecular Biology , National Research Institute of Animal Production , Balice n. Kraków , Poland

2. 2 Center for Experimental and Innovative Medicine , University of Agriculture in Krakow , Rędzina 1c, 30-248 Kraków , Poland

3. 3 Department of Animal Anatomy and Preclinical Sciences, University Centre of Veterinary Medicine JU-UA , University of Agriculture in Krakow , Mickiewicza 24/28, 30-059 Kraków , Poland

4. 4 University Centre of Veterinary Medicine JU-UA, University of Agriculture in Krakow , Mickiewicza 24/28 , Kraków , Poland

5. 5 Equine Vet Clinic EQUI-VET , Stogniowice 55A , Stogniowice , Poland

6. 6 Equine Hospital on the Racing Truck , Służewiec, Puławska 266 , Warszawa , Poland

Abstract

Abstract Sarcoids are the most common dermatological tumor of equids worldwide. These fibroblastic tumors rarely regress, more often they remain and can be locally invasive. To explore the functional importance of DNA methylation during the BPV-dependent neoplasia of equine dermal tissues, this study assessed a methylation level of CpG islands (CGIs) in the promoter region of LPAR1, MPZL3, and POPDC2 genes, and within the gene body of the EHF gene, which were found to be differentially expressed in sarcoid samples in a previous study and harbored the differentially methylated sites (DMSs) in their sequences. The results of bisulfite sequencing PCR (BSP) showed a decrease in the methylation levels of CGIs of LPAR1 and EHF genes and an increase in the case of POPDC2. The obtained data did not reveal any changes in the level of DNA methylation in the analyzed promoter region of the MPZL3 gene between the tumor and healthy tissues. Our observations confirm the presence of CpG methylation alterations in the equine sarcoid and provide new insight into a better understanding of the epigenetic regulation of this tumor development.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/aoas-2023-0078

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