The participation of ribosome-UDP-GalNAc complex in the initiation of protein glycosylation in vitro.
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Published:2000-06-30
Issue:2
Volume:47
Page:421-426
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ISSN:1734-154X
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Container-title:Acta Biochimica Polonica
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language:
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Short-container-title:Acta Biochim Pol
Author:
Paszkiewicz-Gadek A,Porowska H,Gindzieński A
Abstract
The gastric epithelial cells ribosome-UDP-GalNAc complex is a donor of UDP-GalNAc as the substrate for N-acetylgalactosaminyltransferase, which catalyse the transfer of GalNAc residue to the polypeptide, existing on polysomes. It was observed that the deglycosylated porcine mucin and synthetic peptide (PTSSPIST) can be also glycosylated with participation of N-acetylgalactosaminyltransferase and ribosome-UDP-GalNAc complex. The probability of the ribosome-UDP-GalNAc complex as an intermediate in the O-glycosylation is considered.
Publisher
Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)
Subject
General Biochemistry, Genetics and Molecular Biology