Effect of bioactive glass-based composite and low energylaser on bone regeneration in an experimentally induced bone defect

Abstract

In vivo experimental study was conducted to investigate the effect of bone regeneration under the use of a bioactive glass-based composite as well as the use of laser irradiation in the presence of the bioactive glass-based composite. In 16 white non-inbred rats, with a body weight of 300–350 g the bioactive glass-based composite was implanted into the tibial metaphysis. Besides, 90 female rats of the Wistar line weighing 250–270 g underwent laser irradiation on an artificially created tibial bone defect filled with the bioactive glass-based composite. The period of observation lasted respectively 2 and 4 weeks. It was found that application of the bioactive glass promotes bone regeneration, which is noticeably enhanced with the use of low energy laser. The combining effect leads to a significant increase in bone regeneration and the expression of bone tissue metabolic markers: osteocalcin and alkaline phosphatase. The most pronounced effect of the laser on bone regeneration occurred at a wavelength of 810 nm, which opens up prospects for the use of this technique in clinical practice.