CLONAL REPRODUCTION OF VACCINIUM CORYMBOSUM L. OF BLUE BERRY VARIETY IN CULTURE OF TISSUES-Reference-Cited by-同舟云学术

CLONAL REPRODUCTION OF VACCINIUM CORYMBOSUM L. OF BLUE BERRY VARIETY IN CULTURE OF TISSUES

Published:2019-07-29 Issue:2 Volume:14 Page:20-25
ISSN:2073-0462
Container-title:Vestnik of Kazan State Agrarian University
language:en
Short-container-title:

Author:

Мохамед Гамил¹,Mohamed Gamil²,Хуснетдинова Ландыш¹,Husnetdinova Landysh²,Тимофеева Ольга¹,Timofeeva Ol'ga²

Affiliation:

1. Казанский (Приволжский) федеральный университет

2. Kazan (Volga) Federal University

Abstract

For efficient clonal reproduction of Vaccinium corymbosum L. of Blue Berry variety, the surface explant sterilization approaches have been developed - the apical part of the stem and stem segments using “Belizna” commercial bleach solution and nutrient media for explant cultivation have been selected. The maximum survival of the explants (100%) was observed during sterilization with a 15% of “Belizna” bleach solution with an exposure time of 15 minutes. The nutrient media Murasige-Skuga, WPM and Anderson were compared for effective breeding. The highest breeding rate of blueberry was on WPM medium with the addition of zeatin and indolyl-3-butyric acid. The maximum number of axillary shoots per explant was 3.80, with an average length of 3.26 cm, obtained on WPM medium containing 1.0 mg/l of zeatin and 0.1 mg/l of indolyl-3-butyric acid. It has been shown that with an increase in the number of passages, growth rates improve. The maximum increase in the number of healthy axillary shoots is observed at the fourth passage, while the phenomenon of vitrification begins to appear from the fifth passage.

Publisher

Infra-M Academic Publishing House

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