Abstract
Callus culture, an alternative technique for the production of secondary metabolites, is a plant tissue culture method. In this study, it was aimed to determine the anti-alzheimer and anti-diabetic activity of ethanol extracts of callus tissue obtained from the Edirne hyacinth (Bellevalia edirnensis ÖZHATAY & MATHEW), which is endemic and is in danger of extinction. Callus induction started by sterilization of the seeds in 20% bleach and 70% ethanol. Following, it was achieved by culturing in MS medium containing (Murashige ve Skoog) GA3 in 3 different concentrations in addition to the medium supplemented with 7 g/L agar and 30 g/L sucrose. Anti-Alzheimer activity of the obtained calluses were determined using the Ellman method which is a spectrophotometric method. Anti-diabetic activities were determined using the α-glucosidase inhibition method. According to the results obtained, it was found that the callus culture of Bellevalia edirnensis ÖZHATAY & MATHEW showed moderate Anti-Alzheimer and Anti-diabetic activity.
Publisher
Kirklareli Universitesi Muhendislik ve Fen Bilimleri Dergisi
Reference21 articles.
1. Mazid, M., Khan, T., & Mohammad F. (2011). Role of secondary metabolites in defence mechanisms of plants. Biology and Med., 3(2), 232-249.
2. Ceylan, B., Tırıs, G. & Kepekci Tekkeli E. (2021). A new HPLC method with UV detection for the determination of carnosol in human plasma and application to a pharmacokinetic study. Chromatoraphia., 89(9), 855-860.
3. Elansary, H.O., Szopa, A., Kubica, P., Al-Mana, F.A., Mahmoud, E.A., El-Abedin, T.K.A.Z., Mattar, M.A., & Ekiert, H. (2019). Pheolic compounds of Catalpa speciose Taxus cuspidate, and Magnolia acuminata have antioxidant and anticancer activity. Molecules, 24(3), 412.
4. Serim, E., Ceylan, B., & Tekkeli Kepekci S.E. (2023). Determination of apigenin in cosmetics containing chamomile by High-Performance Liquid Chromatography with Ultraviolet Detection (HPLC-UV). Analytical Letters, 56(13), 2113-2122.
5. Ceylan, B., & Yeşiloğlu Y. (2022). Investigation of antioxidant activity of Sorbus domestica L. extracts and determination of phenolic contents by LC-MS/MS. Revue Roumaine De Chimie, 67(6-7), 343-351.