Abstract
Background: Nowadays, despite the existence of genomics and proteomics, numerous genes and proteins have been discovered; however, the function and role of most of them are still unknown. Using bioinformatics tools can be a major step in the identification of these proteins. Methods: This study used various bioinformatics software to identify the unknown protein properties with the AT2G15110.1 accession number on the Arabidopsis Information Resource website. Operations, such as the identification of general protein properties, blasting amino acid sequences, the identification of motifs and domains in the sequence, the examination of the second and third protein structures, the identification of ligands, the identification of proteins involved with the protein, and the identification of the protein site in the cell, were carried out. Results: The results showed that the query protein had no significant homology in terms of sequence, three-dimensional structure, and any interaction with known proteins. Additionally, it was observed that the presence probability of this protein in the nucleus organelle was more than in other organelles, and it only has one domain of unknown function. Conclusions: The results of this study would be basic information for other researchers who seek to identify this unknown protein and determine the right pathway for the identification of mentioned protein function through bioinformatics tools or laboratory methods. Based on the results of this study, laboratory methods are recommended for subsequent studies.