Abstract
Objectives: Bread has long been one of the most popular foods and is the major source to supply energy, protein, minerals, and vitamins. The type of bread making process may affect its nutritional value. This study aimed to investigate bread baking methods and their effects on protein efficacy ratio (PER) and the status of some iron-related blood parameters in rats. Methods: Four diets were used in this study, including a control diet (animals chow), fermented bread, non-fermented bread, and soda bread. At first, some chemical parameters of flour and bread were measured, and then PER and some hematological parameters were determined in rats. Descriptive statistics include mean ± standard deviation (SD), and analytical statistics include one-way ANOVA and Tukey post hoc test. P < 0.05 was considered statistically significant. Results: The wheat flour’s Fe was 18.68 mg/kg. The PER value was negative in the group that received soda bread, and the weight gain was the lowest in the soda bread group. Ferritin, RBC, Hematocrit, BUN, MCV, and MCHC mean values were significantly lower in the rats that received soda bread than other groups. There were no differences among groups in MCH. Conclusions: Although bread can be used as a major source to supply energy protein and other nutritional values, the soda bread making process may decrease protein efficacy ratio and cause debilitation of hematological parameters. These effects of soda can disrupt the body's physiological processes and lead to disease in the long run.