Affiliation:
1. Faculty of Pharmacy, Integral University, Lucknow, India
Abstract
Abstract
Background An RP-HPLC (Reverse Phase-High-performance liquid
chromatography) method for the quantitative estimation and validation of the
plumbagin in the methanolic fraction of Plumbago zeylanica L. was
developed.
Method For achieving good separation, the RP-HPLC method was carried out
with reverse phase C18 column, using methanol and water as mobile phase in the
ratio of 65:35 (v/v), at the flow rate of 1 mL/min. The
detection wavelength was set at 265 nm.
Results The retention time of plumbagin was found at
7.5±0.2 min. The coefficient of determination of plumbagin was
found to be (r2) 0.9985 and equation Y=23148x+4327.
The LOD and LOQ were found to be 34.06 and 113 ng/mL,
respectively.
Conclusion The developed method was accurate, specific, precise, and
reproducible. This RP-HPLC may be useful for quantitative estimation of the
chemical constituents present in the plant extract as well as the quality
assessment of the herbal product.
Subject
Drug Discovery,General Medicine
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