Affiliation:
1. Faculty of Agricultural Science and Food Industries, Science and
Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Georgia State University, Atlanta, GA,
USA
Abstract
Abstract
Cannabis sativa L. is an annual dioecious plant that belongs to the
Cannabaceae family and is essential for different pharmaceutical and nutritional
properties. The most important and prevalent cannabinoids in cannabis are
cannabidiol and delta-9-tetrahydrocannabinol. The application of elicitors is an
effective method to improve secondary metabolite production, leading to a whole
spectrum of molecular, genetic, and physiological modifications. Therefore, the
expression changes of four key genes (THCAS, CBDAS, PT, and
OLS) of the cannabinoids pathway along with the
delta-9-tetrahydrocannabinol and cannabidiol metabolites fluctuation were
surveyed following the application of ascorbic acid as an elicitor. Cannabis was
sprayed immediately before flowering with ascorbic acid. Treated and untreated
(control) plants were sampled in different time courses for real-time PCR and
HPLC experiments. Results showed significant increases in THCAS,
CBDAS, PT, and OLS expression after ascorbic acid
treatments. The results of metabolite quantification also indicated that
secondary metabolites, especially delta-9-tetrahydrocannabinol and cannabidiol,
increased after the ascorbic acid application. This study contributes to the
growing body of knowledge of the functions of key genes in the cannabinoids
pathway to the engineering of cannabis for improving the production of
delta-9-tetrahydrocannabinol and cannabidiol metabolites in this plant.
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