EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress

Author:

Peslak Scott A.12,Wenger Jesse1,Bemis Jeffrey C.3,Kingsley Paul D.1,Koniski Anne D.1,McGrath Kathleen E.1,Palis James1

Affiliation:

1. Center for Pediatric Biomedical Research, Department of Pediatrics, and

2. Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY; and

3. Litron Laboratories, Rochester, NY

Abstract

AbstractErythropoiesis is a robust process of cellular expansion and maturation occurring in murine bone marrow and spleen. We previously determined that sublethal irradiation, unlike bleeding or hemolysis, depletes almost all marrow and splenic erythroblasts but leaves peripheral erythrocytes intact. To better understand the erythroid stress response, we analyzed progenitor, precursor, and peripheral blood compartments of mice post–4 Gy total body irradiation. Erythroid recovery initiates with rapid expansion of late-stage erythroid progenitors–day 3 burst-forming units and colony-forming units, associated with markedly increased plasma erythropoietin (EPO). Although initial expansion of late-stage erythroid progenitors is dependent on EPO, this cellular compartment becomes sharply down-regulated despite elevated EPO levels. Loss of EPO-responsive progenitors is associated temporally with a wave of maturing erythroid precursors in marrow and with emergence of circulating erythroid progenitors and subsequent reestablishment of splenic erythropoiesis. These circulating progenitors selectively engraft and mature in irradiated spleen after short-term transplantation, supporting the concept that bone marrow erythroid progenitors migrate to spleen. We conclude that sublethal radiation is a unique model of endogenous stress erythropoiesis, with specific injury to the extravascular erythron, expansion and maturation of EPO-responsive late-stage progenitors exclusively in marrow, and subsequent reseeding of extramedullary sites.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Reference56 articles.

1. Periodicity during recovery of erythropoiesis following irradiation.;Morley;Blood,1969

2. The effect of sublethal x-irradiation on erythropoiesis in the mouse.;Brady;Int J Radiat Oncol Biol Phys,1976

3. Extended recovery lag in rat hematopoietic colony forming units following sublethal x-irradiation.;Okunewick;J Lab Clin Med,1972

4. Ultrastructural studies of erythroblastic islands of rat bone marrow. 3. Effects of sublethal irradiation.;Ben-Ishay;Lab Invest,1974

5. Response of Megakaryocyte, Erythroid, and Granulocyte-Macrophage Progenitor Cells in Mouse Bone Marrow to Gamma-Irradiation and Cyclophosphamide.;Nakeff,1979

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