Affiliation:
1. Division of Medical Oncology, Department of Oncology;
2. Division of Hematology, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, MN
Abstract
Abstract
Chaetocin, a thiodioxopiperazine natural product previously unreported to have anticancer effects, was found to have potent antimyeloma activity in IL-6–dependent and –independent myeloma cell lines in freshly collected sorted and unsorted patient CD138+ myeloma cells and in vivo. Chaetocin largely spares matched normal CD138− patient bone marrow leukocytes, normal B cells, and neoplastic B-CLL (chronic lymphocytic leukemia) cells, indicating a high degree of selectivity even in closely lineage-related B cells. Furthermore, chaetocin displays superior ex vivo antimyeloma activity and selectivity than doxorubicin and dexamethasone, and dexamethasone- or doxorubicin-resistant myeloma cell lines are largely non–cross-resistant to chaetocin. Mechanistically, chaetocin is dramatically accumulated in cancer cells via a process inhibited by glutathione and requiring intact/unreduced disulfides for uptake. Once inside the cell, its anticancer activity appears mediated primarily through the imposition of oxidative stress and consequent apoptosis induction. Moreover, the selective antimyeloma effects of chaetocin appear not to reflect differential intracellular accumulation of chaetocin but, instead, heightened sensitivity of myeloma cells to the cytotoxic effects of imposed oxidative stress. Considered collectively, chaetocin appears to represent a promising agent for further study as a potential antimyeloma therapeutic.
Publisher
American Society of Hematology
Subject
Cell Biology,Hematology,Immunology,Biochemistry
Reference36 articles.
1. Hauser D, Weber HP, Sigg HP. Isolation and configuration of Chaetocin. Helv Chim Acta1970; 53:1061–1073.
2. Weber HP. The molecular structure and absolute configuration of chaetocin. Acta Cryst1972; B28:2945–2951.
3. Katagiri K, Sato K, Hayakawa S, Matsushima T, Minato H. Verticillin A, a new antibiotic from Verticillium sp. J Antibiot (Tokyo)1970; 23:420–422.
4. Dong JY, He HP, Shen Y-M, Zhang K-Q. Nematicidal Epipolysulfany-ldioxopiperazines from Gliocladium roseum. J Nat Prod2005; 68:1510–1513.
5. Neuss N, Boeck LD, Brannon DR, et al. Aranotin and related metabolites from Arachniotus aureus (Eidam) Schroeter, IV: fermentation, isolation, structure elucidation, biosynthesis, and antiviral properties. Antimicrobial Agents Chemother1968; 8:213–219.
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