Identification of factors promoting ex vivo maintenance of mouse hematopoietic stem cells by long-term single-cell quantification-Reference-Cited by-同舟云学术

Identification of factors promoting ex vivo maintenance of mouse hematopoietic stem cells by long-term single-cell quantification

Published:2016-09-01 Issue:9 Volume:128 Page:1181-1192
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

Kokkaliaris Konstantinos D.¹²^ORCID,Drew Erin¹,Endele Max¹²,Loeffler Dirk¹²,Hoppe Philipp S.¹²,Hilsenbeck Oliver¹²,Schauberger Bernhard¹,Hinzen Christoph³,Skylaki Stavroula¹²,Theodorou Marina⁴,Kieslinger Matthias³,Lemischka Ihor⁵,Moore Kateri⁵,Schroeder Timm¹²

Affiliation:

1. Research Unit Stem Cell Dynamics, Helmholtz Center Munich–German Research Center for Environmental Health (GmbH), Neuherberg, Germany;

2. Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland;

3. Institute of Clinical Molecular Biology and Tumor Genetics, Helmholtz Center Munich–German Research Center for Environmental Health (GmbH), Munich, Germany;

4. Institute for Developmental Genetics, Helmholtz Center Munich–German Research Center for Environmental Health (GmbH), Neuherberg, Germany; and

5. Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY

Abstract

Key Points AFT024-induced HSC maintenance correlates with early survival/proliferation whereas early death is a major reason for HSC loss in culture. Dermatopontin is required for ex vivo HSC maintenance, and also improves HSC clonogenicity in stroma-based and stroma-free cultures.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/128/9/1181/1398824/1181.pdf

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