Increase in the specificity of immunoassay methods by direct targeting different epitopes; sequential chain reactions

Abstract

BACKGROUND: Immunoassay methods typically involve the use of antibodies, which are either labeled with an enzyme to generate a detectable product or directly tagged with a radioactive or fluorescent substrate. METHODS: One approach to enhance the specificity of immuno-detection methods is by employing a combination of different antibodies, such as primary and secondary. RESULTS: However, relying solely on one antibody targeting another may not offer the highest level of precision for improving immunoassay specificity; A novel strategy for enhancing the specificity of immunoassay techniques involves directly targeting different epitopes of an antigen. CONCLUSIONS: This approach entails utilizing sequential chain reactions facilitated by distinct enzymes bound to various antibodies, each directed at specific epitopes on the antigen. Such an innovative method holds promise for advancing the specificity of immunoassay methods.