Development, characterization and anti-Mycobacterium tuberculosisactivity of solid lipid nanoparticles for oral Piper corcovadensisroots extract delivery
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Published:2023-11-30
Issue:6
Volume:22
Page:821-836
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ISSN:0717-7917
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Container-title:Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
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language:
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Short-container-title:BLACPMA
Author:
Fernandez Carla Maria MarianoORCID, , Kleinubing Sirlene AdrianaORCID, Karam Thaysa KsiaskiewczORCID, Lorenzetti Fabiana BruscoORCID, Palomo Carolina TrevisolliORCID, de Oliveira Byanca Pereira MoreiraORCID, Goncalves Odinei HessORCID, Gazim Zilda CristianiORCID, Romagnolo Mariza BarionORCID, Caleffi-Ferracioli Katian y RizzieriORCID, Scodro Regiane Bertin de LimaORCID, Lima Marli Miriam de SouzaORCID, Nakamura Tania UedaORCID, Nakamura Celso VataruORCID, Filho Benedito Prado DiasORCID, , , , , , , , , , , , , ,
Abstract
The present study thus aimed at the development and physicochemical characterization of solid lipid nanoparticles loaded with crude extract of Piper corcovadensisroots (SLN-CEPc) and chitosan-coated solid lipid nanoparticles loaded withcrude extract of P. corcovadensisroots (C-SLN-CEPc), as well as the determination of its antimycobacterial activity against Mycobacterium tuberculosisH37Rv, its cytotoxicity against the Vero cell line and evaluation in the hemolysis assay. Both formulations containing the encapsulated extract showed high encapsulation efficiency, formed by a monodispersed system with small and spherical particles, and there was no aggregation of particles. In the biological assays, SLN-CEPc and C-SLN-CEPc showed promising anti-M. tuberculosisactivity with a minimum inhibitory concentration (MIC) of 12.5 μg/mL, whereas the cytotoxic concentrations obtained at 50% (CC50) in Vero cells were 60.0 and 70.0 μg/mL, respectively. Therefore, nanoencapsulation showed satisfactory results, justifying its usage in the development of new products.
Subject
Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology
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