Thiol–ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis-Reference-Cited by-同舟云学术

Thiol–ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis

Published:2018 Issue:22 Volume:8 Page:11843-11849
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Wang Meng¹²³⁴⁵,Xing Jun¹²³⁴⁵,Sun Yu-Tang¹²³⁴⁵,Guo Ling-Xiang¹²³⁴⁵,Lin Bao-Ping¹²³⁴⁵^ORCID,Yang Hong¹²³⁴⁵^ORCID

Affiliation:

1. School of Chemistry and Chemical Engineering

2. Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research

3. Jiangsu Key Laboratory for Science and Application of Molecular Ferroelectrics

4. Southeast University

5. Nanjing

Abstract

Here we report a strategy to photoimmobilize chymotrypsin onto polysiloxane gels via thiol-ene click chemistry. The polysiloxane-immobilized chymotrypsin shows enhanced catalytic activity, thermal stability and recyclability.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/RA/C7RA13320K

Reference51 articles.

1. Immobilization and stabilization of subtilisin Carlsberg in magnetically-separable mesoporous silica for transesterification in an organic solvent

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