Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing-Reference-Cited by-同舟云学术

Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing

Published:2018 Issue:5 Volume:18 Page:775-784
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Moon Hui-Sung¹²³⁴^ORCID,Je Kwanghwi⁵⁶⁷⁴,Min Jae-Woong¹²³⁴,Park Donghyun¹²³⁴,Han Kyung-Yeon¹²³⁴,Shin Seung-Ho¹²³⁴⁸,Park Woong-Yang¹²³⁴⁸,Yoo Chang Eun¹²³⁴,Kim Shin-Hyun⁵⁶⁷⁴^ORCID

Affiliation:

1. Samsung Genome Institute

2. Samsung Medical Center

3. Seoul 06351

4. South Korea

5. Department of Chemical and Biomolecular Engineering

6. Korea Advanced Institute of Science and Technology (KAIST)

7. Daejeon

8. Department of Health Sciences and Technology

Abstract

We developed a modified high-throughput droplet barcoding technique for single-cell Drop-Seq via introduction of hydrodynamic ordering in a spiral microchannel.

Funder

National Research Foundation of Korea

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2018/LC/C7LC01284E

Reference44 articles.

1. Cellular Heterogeneity: Do Differences Make a Difference?

2. Single-cell RNA sequencing identifies distinct mouse medial ganglionic eminence cell types

3. Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types