Analyzing protein–protein interactions in rare cells using microbead-based single-molecule pulldown assay-Reference-Cited by-同舟云学术

Analyzing protein–protein interactions in rare cells using microbead-based single-molecule pulldown assay

Published:2021 Issue:16 Volume:21 Page:3137-3149
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Zhao Qirui¹²³,Shen Yusheng⁴²³,Li Xiaofen⁴²³,Tian Fang⁵²³,Yu Xiaojie¹²³,Yobas Levent⁴²³⁶²^ORCID,Park Hyokeun¹²³⁵²,Duan Yuanyuan⁷⁸⁹¹⁰³,Huang Pingbo¹²³⁴²^ORCID

Affiliation:

1. Division of Life Science

2. Hong Kong University of Science and Technology

3. China

4. Department of Chemical and Biological Engineering

5. Department of Physics

6. Department of Electronic and Computer Engineering

7. Biomedical Translational Research Institute

8. Faculty of Medical Science

9. Jinan University

10. Guangzhou

Abstract

Protein complexes were captured by antibody modified agarose microbeads and detected by corresponding fluorophore-labeled antibodies. This assay is simple and highly sensitive for studying protein–protein interactions in rare cells.

Funder

Research Grants Council, University Grants Committee

Innovation and Technology Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D1LC00260K

Reference23 articles.

1. Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

2. Probing cellular protein complexes using single-molecule pull-down