Fluorescence fluctuation of an antigen–antibody complex: circular dichroism, FCS and smFRET of enhanced GFP and its antibody-Reference-Cited by-同舟云学术

Fluorescence fluctuation of an antigen–antibody complex: circular dichroism, FCS and smFRET of enhanced GFP and its antibody

Published:2015 Issue:38 Volume:17 Page:25250-25259
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Bhunia Debmalya¹²³⁴,Chowdhury Rajdeep⁵⁶³⁴,Bhattacharyya Kankan⁵⁶³⁴,Ghosh Surajit¹²³⁴⁷

Affiliation:

1. Organic & Medicinal Chemistry Division

2. CSIR-Indian Institute of Chemical Biology

3. Jadavpur

4. India

5. Department of Physical Chemistry

6. Indian Association for the Cultivation of Science

7. Academy of Scientific and Innovative Research (AcSIR)

Abstract

The structure and dynamics of an antibody (anti-EGFP V_HH), antigen (EGFP) and their complex are studied using circular dichroism, fluorescence correlation spectroscopy and smFRET.

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2015/CP/C5CP04908C

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