Simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine at specific genomic loci by engineered deaminase-assisted sequencing-Reference-Cited by-同舟云学术

Simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine at specific genomic loci by engineered deaminase-assisted sequencing

Published:2024 Issue:26 Volume:15 Page:10073-10083
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Xie Neng-Bin¹²³,Wang Min⁴⁵,Ji Tong-Tong⁴,Guo Xia⁴,Gang Fang-Yin¹,Hao Ying⁴,Zeng Li⁴,Wang Ya-Fen¹,Feng Yu-Qi¹^ORCID,Yuan Bi-Feng¹²³⁴^ORCID

Affiliation:

1. Department of Occupational and Environmental Health, School of Public Health, Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China

2. Research Center of Public Health, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China

3. Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center, Zhongnan Hospital of Wuhan University, Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan 430071, China

4. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

5. College of Chemical Engineering and Environmental Chemistry, Weifang University, Weifang, 261061, China

Abstract

We developed the EDA-seq method, which enables the simultaneous and quantitative detection of C, 5mC, and 5hmC in DNA at single-base resolution.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Fundamental Research Funds for the Central Universities

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SC/D4SC00930D