Ultrasensitive quantification of PD-L1+ extracellular vesicles in melanoma patient plasma using a parallelized high throughput droplet digital assay-Reference-Cited by-同舟云学术

Ultrasensitive quantification of PD-L1+ extracellular vesicles in melanoma patient plasma using a parallelized high throughput droplet digital assay

Published:2024 Issue:14 Volume:24 Page:3403-3411
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Shen Hanfei¹^ORCID,Atiyas Yasemin¹,Yang Zijian²,Lin Andrew A.¹,Yang Jingbo³,Liu Diao³,Park Juhwan¹,Guo Wei³,Issadore David A.¹⁴^ORCID

Affiliation:

1. Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA

2. Department of Radiology, School of Medicine, Stanford University, Stanford, California, USA

3. Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA

4. Department of Electrical and Systems Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA

Abstract

We develop a high-throughput droplet-based extracellular vesicle analysis (DEVA) assay for ultrasensitive quantification of EVs in plasma that are positive for both PD-L1 and tetraspanins.

Funder

National Institute of Mental Health

National Institutes of Health

National Human Genome Research Institute

National Institute of Allergy and Infectious Diseases

National Cancer Institute

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/LC/D4LC00331D

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