A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis-Reference-Cited by-同舟云学术

A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis

Published:2017 Issue:21 Volume:142 Page:4030-4038
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Hirose Kazuki¹²³⁴,Tsuchida Maho¹²³⁴,Asakura Hinako¹²³⁴,Wakui Koji⁵⁶⁷⁸⁴,Yoshimoto Keitaro⁵⁶⁷⁸⁴,Iida Keisuke¹²³⁴,Sato Makoto⁹¹⁰¹¹⁴,Shibukawa Masami¹²³⁴,Suganuma Masami¹²³⁴¹²,Saito Shingo¹²³⁴^ORCID

Affiliation:

1. Graduate School of Science and Engineering

2. Saitama University

3. Saitama 338-8570

4. Japan

5. Department of Life Sciences

6. Graduate School of Arts and Sciences

7. The University of Tokyo

8. Tokyo

9. Sagamihara R&D Center

10. Shino-Test Corporation

11. Sagamihara 252-0331

12. Research Institute for Clinical Oncology

Abstract

A single-round DNA aptamer selection for mammalian cells was successfully achieved for the first time using a capillary electrophoresis (CE)-based methodology.

Funder

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/AN/C7AN00909G

Reference63 articles.

1. SELEX—A (r)evolutionary method to generate high-affinity nucleic acid ligands

2. Capillary electrophoretic development of aptamers for a glycosylated VEGF peptide fragment

3. A novel protocol for generating high-affinity ssDNA aptamers by using alternating magnetic fields