DNAzyme-RCA-based colorimetric and lateral flow dipstick assays for the point-of-care testing of exosomal m5C-miRNA-21

Author:

Zhang Hao1,Tang Yue2,Zhou Yingshun1,Wang Yiguo3,Si Haibin1,Li Lu1,Tang Bo14ORCID

Affiliation:

1. Department College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, 250014, P. R. China

2. Department of Emergency Medicine, Shandong Provincial Clinical Research Center for Emergency and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, 250014, P. R. China

3. First Affiliated Hospital of Shandong First Medical University, Shandong Provincial Qianfoshan Hospital, Jinan, 250014, P. R. China

4. Laoshan Laboratory, Qingdao 266237, P. R. China

Abstract

The DNAzyme-RCA-based AuNP colorimetric and lateral flow dipstick (LFD) assays were developed for the point-of-care testing of exosomal m5C-miRNA in blood samples from colorectal cancer patients.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shandong Province

Publisher

Royal Society of Chemistry (RSC)

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