Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

Author:

Weissenborn Martin J.1234,Notonier Sandra1234,Lang Sarah-Luise1234,Otte Konrad B.1234,Herter Susanne56789,Turner Nicholas J.56789,Flitsch Sabine L.56789,Hauer Bernhard1234

Affiliation:

1. Institute of Technical Biochemistry

2. Universitaet Stuttgart

3. 70569 Stuttgart

4. Germany

5. School of Chemistry

6. University of Manchester

7. Manchester Institute of Biotechnology

8. M1 7DN Manchester

9. UK

Abstract

A whole cell screening assay for terminal hydroxylases was developed based on a readily available galactose oxidase enzyme.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Reference34 articles.

1. Improved biocatalysts by directed evolution and rational protein design

2. T. W. Johannes , R. D.Woodyer and H.Zhao, Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting, 2006, pp. 77–93

3. Colorimetric High-Throughput Assay for Alkene Epoxidation Catalyzed by Cytochrome P450 BM-3 Variant 139-3

4. Cytochromes P450 as versatile biocatalysts

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