Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis-Reference-Cited by-同舟云学术

Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis

Published:2019 Issue:24 Volume:19 Page:4016-4020
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Godino Neus¹²³,Pfisterer Felix¹²³,Gerling Tobias¹²³,Guernth-Marschner Christian¹²³,Duschl Claus¹²³,Kirschbaum Michael¹²³^ORCID

Affiliation:

1. Fraunhofer IZI-BB

2. 14476 Potsdam

3. Germany

Abstract

We employ real-time image processing in the active control of dielectrophoretic actuation to select, isolate and arrange individual cells in a microfluidic channel.

Funder

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2019/LC/C9LC00800D

Reference26 articles.

1. Profiling lymphocyte interactions at the single-cell level by microfluidic cell pairing

2. Profiling Functional and Biochemical Phenotypes of Circulating Tumor Cells Using a Two-Dimensional Sorting Device

3. An Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells

4. Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay

5. Single-cell analysis reveals that stochasticity and paracrine signaling control interferon-alpha production by plasmacytoid dendritic cells

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