Exponential magnetophoretic gradient for the direct isolation of basophils from whole blood in a microfluidic system-Reference-Cited by-同舟云学术

Exponential magnetophoretic gradient for the direct isolation of basophils from whole blood in a microfluidic system

Published:2022 Issue:9 Volume:22 Page:1690-1701
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Castaño Nicolas¹^ORCID,Kim Sungu¹^ORCID,Martin Adrian M.¹^ORCID,Galli Stephen J.²³^ORCID,Nadeau Kari C.⁴⁵^ORCID,Tang Sindy K. Y.¹^ORCID

Affiliation:

1. Department of Mechanical Engineering, Stanford University, USA

2. Department of Pathology, Stanford University, USA

3. Department of Microbiology and Immunology, Stanford University, USA

4. Department of Medicine and Pediatrics, with courtesy in Otolaryngology and in Population Science and Epidemiology, Stanford University, USA

5. Sean N. Parker Center for Allergy and Asthma Research, Stanford University, USA

Abstract

We developed a microfluidic platform for rapid immunomagnetic isolation of basophils directly from whole blood. Our 3D printed magnetic separation device imparts an exponentially increasing magnetic force field to remove tagged non-basophils.

Funder

Parker Foundation

National Institute of Allergy and Infectious Diseases

Crown Family Philanthropies

Stanford Bio-X

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D2LC00154C

Reference78 articles.

1. Cytokine production by mast cells and basophils

2. Multifaceted roles of basophils in health and disease

3. The Evolution of Human Basophil Biology from Neglect towards Understanding of Their Immune Functions

4. Emerging roles of basophils in allergic inflammation

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