Analysis of protein phosphorylation in solution and in cells by using an ATP analogue in combination with fluorescence techniques-Reference-Cited by-同舟云学术

Analysis of protein phosphorylation in solution and in cells by using an ATP analogue in combination with fluorescence techniques

Published:2021 Issue:14 Volume:146 Page:4506-4514
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Li Yue¹²³⁴⁵,Huang Xiangyi¹²³⁴⁵^ORCID,Ren Jicun¹²³⁴⁵^ORCID

Affiliation:

1. School of Chemistry and Chemical Engineering

2. State Key Laboratory of Metal Matrix Composites

3. Shanghai Jiao Tong University

4. Shanghai

5. P. R. China

Abstract

We synthesized a new ATP analogue ATP-NB, and a fluorescent probe TZ-Cy3 based on D–A reaction. Using fluorescent techniques, we established an efficient method for studying the phosphorylation of proteins in solution and in living cells.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shanghai

Shanghai Key Basic Research Program

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00742D

Reference46 articles.

1. Protein post-translational modifications in bacteria

2. Rare Earth Ion Mediated Fluorescence Accumulation on a Single Microbead: An Ultrasensitive Strategy for the Detection of Protein Kinase Activity at the Single-Cell Level

3. In situ study of RSK2 kinase activity in a single living cell by combining single molecule spectroscopy with activity-based probes

4. Simple and Sensitive Method for Determination of Protein Kinase Activity Based on Surface Charge Change of Peptide-Modified Gold Nanoparticles As Substrates

5. Metabolomic analysis of human oral cancer cells with adenylate kinase 2 or phosphorylate glycerol kinase 1 inhibition

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