Analysis of protein phosphorylation in solution and in cells by using an ATP analogue in combination with fluorescence techniques
Author:
Affiliation:
1. School of Chemistry and Chemical Engineering
2. State Key Laboratory of Metal Matrix Composites
3. Shanghai Jiao Tong University
4. Shanghai
5. P. R. China
Abstract
We synthesized a new ATP analogue ATP-NB, and a fluorescent probe TZ-Cy3 based on D–A reaction. Using fluorescent techniques, we established an efficient method for studying the phosphorylation of proteins in solution and in living cells.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Shanghai
Shanghai Key Basic Research Program
Publisher
Royal Society of Chemistry (RSC)
Subject
Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00742D
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4. Simple and Sensitive Method for Determination of Protein Kinase Activity Based on Surface Charge Change of Peptide-Modified Gold Nanoparticles As Substrates
5. Metabolomic analysis of human oral cancer cells with adenylate kinase 2 or phosphorylate glycerol kinase 1 inhibition
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