Indigo production identifies hotspots in cytochrome P450 BM3 for diversifying aromatic hydroxylation-Reference-Cited by-同舟云学术

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Indigo production identifies hotspots in cytochrome P450 BM3 for diversifying aromatic hydroxylation

Published:2024 Issue: Volume:252 Page:29-51
ISSN:1359-6640
Container-title:Faraday Discussions
language:en
Short-container-title:Faraday Discuss.

Author:

Fansher Douglas J.¹²³^ORCID,Besna Jonathan N.²³⁴^ORCID,Pelletier Joelle N.¹²³⁴^ORCID

Affiliation:

1. Chemistry Department, Université de Montréal, Montreal, QC, Canada

2. PROTEO, The Québec Network for Research on Protein, Function, Engineering and Applications, Quebec, QC, Canada

3. CGCC, Center in Green Chemistry and Catalysis, Montreal, QC, Canada

4. Department of Biochemistry and Molecular Medicine, Université de Montréal, Montreal, QC, Canada

Abstract

Indigo (+) and indigo (−) single variants and a combinatorial library, with mutations that enable the blue phenotype, were screened for their ability to hydroxylate a panel of 12 aromatic compounds using the 4-aminoantipyrine colorimetric assay.

Funder

Canada Research Chairs

Natural Sciences and Engineering Research Council of Canada

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/FD/D4FD00017J

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3. An overview of enzymatic production of biodiesel

4. Biocatalysis: Enzymatic Synthesis for Industrial Applications

5. Chemo-enzymatic synthesis of pharmaceutical intermediates

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