Cluster-selective <sup>57</sup>Fe labeling of a Twitch-domain-containing radical SAM enzyme-Reference-Cited by-同舟云学术

Cluster-selective ⁵⁷Fe labeling of a Twitch-domain-containing radical SAM enzyme

Published:2023 Issue:27 Volume:14 Page:7492-7499
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Namkoong Gil¹^ORCID,Suess Daniel L. M.¹^ORCID

Affiliation:

1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Abstract

Although the radical SAM enzyme BtrN contains two [Fe4S4] clusters, it is shown that only one undergoes exchange with exogenous Fe ions. This enables the facile and selective 57Fe labeling of either [Fe4S4] cluster.

Funder

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D3SC02016A

Reference43 articles.

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4. An Anchoring Role for FeS Clusters: Chelation of the Amino Acid Moiety of S-Adenosylmethionine to the Unique Iron Site of the [4Fe−4S] Cluster of Pyruvate Formate-Lyase Activating Enzyme

5. Coordination of Adenosylmethionine to a Unique Iron Site of the [4Fe-4S] of Pyruvate Formate-Lyase Activating Enzyme: A Mössbauer Spectroscopic Study

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