Centrifugal microfluidic lab-on-a-chip system with automated sample lysis, DNA amplification and microarray hybridization for identification of enterohemorrhagic Escherichia coli culture isolates

Author:

Geissler Matthias1234ORCID,Brassard Daniel1234ORCID,Clime Liviu1234ORCID,Pilar Ana Victoria C.5674,Malic Lidija1234ORCID,Daoud Jamal1234,Barrère Virginie5674,Luebbert Christian5674,Blais Burton W.8974,Corneau Nathalie5674,Veres Teodor1234

Affiliation:

1. Life Sciences Division

2. National Research Council of Canada

3. Boucherville

4. Canada

5. Health Canada

6. Bureau of Microbial Hazards

7. Ottawa

8. Ontario Laboratory Network

9. Canadian Food Inspection Agency

Abstract

Automated workflow that starts with a colony isolate and ends with a fluorescence signal on a DNA microarray.

Funder

National Research Council Canada

Health Canada

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference76 articles.

1. Food Safety , World Health Organization , Geneva, Switzerland , 2019 . Available at https://www.who.int/news-room/fact-sheets/detail/food-safety , accessed September 26, 2019

2. Foodborne Illness Acquired in the United States—Major Pathogens

3. Estimates of Foodborne Illness in the United States , Centers for Disease Control and Prevention , Atlanta, GA, USA , 2011 . Available at https://www.cdc.gov/foodborneburden/index.html , accessed 29 August 2019

4. Economic Burden from Health Losses Due to Foodborne Illness in the United States

5. Diarrheagenic Escherichia coli

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