Distinguishing chemically induced NADPH- and NADH-related metabolic responses using phasor analysis of UV-excited autofluorescence-Reference-Cited by-同舟云学术

Distinguishing chemically induced NADPH- and NADH-related metabolic responses using phasor analysis of UV-excited autofluorescence

Published:2021 Issue:31 Volume:11 Page:18757-18767
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Short Audrey H.¹²³⁴^ORCID,Al Aayedi Nazar¹²³⁴,Gaire Madhu¹²³⁴,Kreider Max¹²³⁴,Wong Chong Kai¹²³⁴,Urayama Paul¹²³⁴^ORCID

Affiliation:

1. Department of Physics

2. Miami University

3. Oxford

4. USA

Abstract

A phasor approach was used to show that chemically induced cellular autofluorescence responses linked to NADPH and NADH pathways can be distinguished.

Funder

Miami University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/RA/D1RA02648H

Reference52 articles.

1. Investigating mitochondrial redox state using NADH and NADPH autofluorescence

2. NAD+/NADH and NADP+/NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences

3. Conformational Dependence of Intracellular NADH on Metabolic State Revealed by Associated Fluorescence Anisotropy*♦

4. Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging

5. In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH

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