Complementary techniques to analyse pericellular matrix formation by human MSC within hyaluronic acid hydrogels-Reference-Cited by-同舟云学术

Complementary techniques to analyse pericellular matrix formation by human MSC within hyaluronic acid hydrogels

Published:2020 Issue:8 Volume:1 Page:2888-2896
ISSN:2633-5409
Container-title:Materials Advances
language:en
Short-container-title:Mater. Adv.

Author:

Salzlechner Christoph¹²³⁴,Walther Anders Runge¹²³⁴⁵,Schell Sophie¹²³⁴⁶,Merrild Nicholas Groth¹²³⁴,Haghighi Tabasom¹²³⁴,Huebscher Isabella¹²³⁴,Undt Gerhard⁷⁸⁹¹⁰¹¹,Fan Kathleen¹²¹³¹⁴⁴,Bergholt Mads Sylvest¹²³⁴,Hedegaard Martin A. B.⁵¹⁵¹⁶¹⁷¹⁸^ORCID,Gentleman Eileen¹²³⁴^ORCID

Affiliation:

1. Centre for Craniofacial and Regenerative Biology

2. King's College London

3. London SE1 9RT

4. UK

5. Department of Chemical Engineering

6. Department of Conservative Dentistry

7. University Clinic of Dentistry

8. Department of Oral Surgery

9. Medical University of Vienna

10. 1090 Vienna

11. Austria

12. Department of Oral and Maxillofacial Surgery

13. King's College Hospital

14. London

15. Biotechnology and Environmental Technology

16. University of Southern Denmark

17. DK-5230 Odense

18. Denmark

Abstract

Hydrogels are used widely for cell encapsulation to mimic the native ECM. Here, we characterise and visualise the matrix secreted by encapsulated cells by combining fluorescent non-canonical amino acid tagging with confocal Raman spectral imaging.

Funder

Rosetrees Trust

Det Frie Forskningsråd

Publisher

Royal Society of Chemistry (RSC)

Subject

Microbiology

Link

http://pubs.rsc.org/en/content/articlepdf/2020/MA/D0MA00472C

Reference48 articles.

1. Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels

2. Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate