Sensitivity of the amide I band to matrix manipulation in bone: a Raman micro-spectroscopy and spatially offset Raman spectroscopy study

Author:

Ahmed Rafay1ORCID,Unal Mustafa23ORCID,Gautam Rekha456ORCID,Uppuganti Sasidhar1ORCID,Derasari Shrey56,Mahadevan-Jansen Anita56ORCID,Nyman Jeffry S.157ORCID

Affiliation:

1. Department of Orthopaedic Surgery, Vanderbilt University Medical Center, 1215 21st Ave. S., Suite 4200, Nashville, TN 37232, USA

2. Department of Bioengineering, Karamanoglu Mehmetbey University, Karaman, Türkiye 70200

3. Department of Biophysics, Faculty of Medicine, Karamanoglu Mehmetbey University, Karaman, Türkiye 70200

4. Biophotonics@Tyndall, IPIC, Tyndall National Institute, Cork, Ireland

5. Department of Biomedical Engineering, Vanderbilt University, 5824 Stevenson Center, Nashville, TN 37232, USA

6. Vanderbilt Biophotonics Center, 410 24th Ave. S., Nashville, TN 37232, USA

7. Department of Veterans Affairs, Tennessee Valley Healthcare System, 1310 24th Ave. S., Nashville, TN 37212, USA

Abstract

The SORS probe and the proposed method successfully detected the effects of high temperature and pressure (autoclaving) on collagen type 1 integrity changes in human cortical bone.

Funder

National Institute of Arthritis and Musculoskeletal and Skin Diseases

U.S. Department of Veterans Affairs

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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