Protein painting for structural and binding site analysis <i>via</i> intracellular lysine reactivity profiling with <i>o</i>-phthalaldehyde-Reference-Cited by-同舟云学术

Protein painting for structural and binding site analysis via intracellular lysine reactivity profiling with o-phthalaldehyde

Published:2024 Issue:16 Volume:15 Page:6064-6075
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Zheng Zhenxiang¹,Zeng Ya¹²,Lai Kunjia¹,Liao Bin¹,Li Pengfei¹,Tan Chris Soon Heng¹^ORCID

Affiliation:

1. Department of Chemistry, College of Science, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China

2. Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, PR China

Abstract

We developed an intracellular chemical covalent labeling method based on lysine reactive shift coupled with a new data analysis strategy RAPID to analyze the intracellular conformational changes of proteins and ligand-binding sites on a proteome scale.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Science, Technology and Innovation Commission of Shenzhen Municipality

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SC/D4SC00032C

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