Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme-Reference-Cited by-同舟云学术

Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme

Published:2023 Issue:13 Volume:23 Page:3016-3033
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Doppler Diandra¹²^ORCID,Sonker Mukul¹²^ORCID,Egatz-Gomez Ana¹²^ORCID,Grieco Alice³,Zaare Sahba²⁴,Jernigan Rebecca¹²,Meza-Aguilar Jose Domingo²,Rabbani Mohammad T.¹²,Manna Abhik¹²^ORCID,Alvarez Roberto C.²⁴,Karpos Konstantinos²⁴,Cruz Villarreal Jorvani¹²^ORCID,Nelson Garrett²⁴,Yang Jay-How¹²,Carrion Jackson¹²,Morin Katherine¹²,Ketawala Gihan K.¹²,Pey Angel L.⁵^ORCID,Ruiz-Fresneda Miguel Angel³^ORCID,Pacheco-Garcia Juan Luis⁶^ORCID,Hermoso Juan A.³^ORCID,Nazari Reza²⁴,Sierra Raymond⁷,Hunter Mark S.⁷,Batyuk Alexander⁷^ORCID,Kupitz Christopher J.⁷,Sublett Robert E.⁷,Lisova Stella⁷,Mariani Valerio⁷,Boutet Sébastien⁷,Fromme Raimund¹²^ORCID,Grant Thomas D.⁸,Botha Sabine²⁴,Fromme Petra¹²,Kirian Richard A.²⁴^ORCID,Martin-Garcia Jose Manuel³^ORCID,Ros Alexandra¹²^ORCID

Affiliation:

1. School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA

2. Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA

3. Department of Crystallography and Structural Biology, Institute of Physical Chemistry Blas Cabrera, Spanish National Research Council (CSIC), Serrano 119, 28006, Madrid, Spain

4. Department of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA

5. Departamento de Química Física, Unidad de Excelencia en Química Aplicada a Biomedicina y Medioambiente e Instituto de Biotecnología, Universidad de Granada, Av. Fuentenueva s/n, 18071, Granada, Spain

6. Departamento de Química Física, Universidad de Granada, Av. Fuentenueva s/n, 18071 Granada, Spain

7. Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, 94025 CA, USA

8. Department of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, SUNY University at Buffalo, 955 Main St, Buffalo, NY, 14203, USA

Abstract

A 3D-printed modular droplet injector successfully delivered microcrystals of human NAD(P)H:quinone oxidoreductase 1 (NQO1) and phycocyanin with electrical stimulation in a serial crystallography experiment at 120 Hz repetition rate.

Funder

National Science Foundation

National Institutes of Health

Comunidad de Madrid

U.S. Department of Energy

European Regional Development Fund

Ministerio de Ciencia, Innovación y Universidades

Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering