Fast immunoassay for microfluidic western blotting by direct deposition of reagents onto capture membrane-Reference-Cited by-同舟云学术

Fast immunoassay for microfluidic western blotting by direct deposition of reagents onto capture membrane

Published:2020 Issue:12 Volume:12 Page:1606-1616
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Arvin Natalie E.¹²³⁴^ORCID,Dawod Mohamed¹²³⁴⁵,Lamb Don T.⁶⁷⁴,Anderson Jon P.⁶⁷⁴,Furtaw Michael D.⁶⁷⁴^ORCID,Kennedy Robert T.¹²³⁴⁸^ORCID

Affiliation:

1. Department of Chemistry

2. University of Michigan

3. Ann Arbor

4. USA

5. Vaccine Analytical R&D

6. LI-COR Biosciences

7. Lincoln

8. Department of Pharmacology

Abstract

To further improve the speed and miniaturization of a complete Western blot, a microscale immunoassay with direct deposition of immunoassay reagents has been developed with the flow deposition of antibodies.

Funder

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AY/D0AY00207K

Reference52 articles.

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

2. Protein purification and analysis: next generation Western blotting techniques

3. Western Blotting Using Capillary Electrophoresis

4. Present state of microchip electrophoresis: State of the art and routine applications

5. Western blotting

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