Sensitive label free imaging of 3D cell models with minimal toxicity using confocal reflectance-Reference-Cited by-同舟云学术

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Sensitive label free imaging of 3D cell models with minimal toxicity using confocal reflectance

Published:2024 Issue: Volume: Page:
ISSN:2047-4830
Container-title:Biomaterials Science
language:en
Short-container-title:Biomater. Sci.

Author:

Taylor Michael A.¹^ORCID,Choi Jung Un Ally¹^ORCID,Muthuswamy Shiva¹,Enriquez Martinez Marco A.¹,Lauko Jan¹,Kijas Amanda W.¹,Rowan Alan E.¹

Affiliation:

1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Queensland 4072, Australia

Abstract

Cells that are cultured in transparent 3D hydrogels can be imaged with high sensitivity and low background using confocal reflectance imaging. This label-free approach allows long-term live imaging with low toxicity and minimal specimen preparation.

Funder

Australian Research Council

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/BM/D4BM00304G

Reference22 articles.

1. The cell as matter: Connecting molecular biology to cellular functions

2. Live-cell imaging

3. Is It Time to Start Transitioning From 2D to 3D Cell Culture?

4. Toxicity of Organic Fluorophores Used in Molecular Imaging: Literature Review

5. Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death

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