Development of an <i>in vitro</i> platform for the analysis of contractile and calcium dynamics in single human myotubes-Reference-Cited by-同舟云学术

Development of an in vitro platform for the analysis of contractile and calcium dynamics in single human myotubes

Published:2024 Issue: Volume: Page:
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Vesga-Castro Camila¹²^ORCID,Mosqueira-Martín Laura³⁴⁵^ORCID,Ubiria-Urkola Paul¹²,Marco-Moreno Pablo³⁴⁵^ORCID,González-Imaz Klaudia³⁴⁵,Rendon-Hinestroza Jorge³⁴,Vallejo-Illarramendi Ainara³⁴⁵^ORCID,Paredes Jacobo¹²

Affiliation:

1. Tecnun School of Engineering, University of Navarra, Manuel de Lardizábal 13, 20018 San Sebastián, Spain

2. Biomedical Engineering Center, University of Navarra, Campus Universitario, 31080, Pamplona, Spain

3. Group of Neuroscience, Department of Pediatrics, Faculty of Medicine and Nursing, Donostia Hospital, UPV/EHU, 20014 San Sebastian, Spain

4. Group of Neuromuscular Diseases, IIS Biogipuzkoa, 20014 San Sebastian, Spain

5. CIBERNED, Ministry of Science and Innovation, Instituto de Salud Carlos III, 28031 Madrid, Spain

Abstract

This 2D human muscle system, μGrooves, promote myoblast alignment and fusion and enhance myotubes differentiation. Robust high-content assays of functional outcomes, contractility and calcium, are made with increased sensitivity and reproducibility.

Funder

Eusko Jaurlaritza

Diputación Foral de Gipuzkoa

Ministerio de Ciencia e Innovación

Euskal Herriko Unibertsitatea

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/LC/D3LC00442B

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