Electroanalysis of pM-levels of urokinase plasminogen activator in serum by phosphorothioated RNA aptamer-Reference-Cited by-同舟云学术

Electroanalysis of pM-levels of urokinase plasminogen activator in serum by phosphorothioated RNA aptamer

Published:2015 Issue:11 Volume:140 Page:3794-3802
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Jarczewska Marta¹²³⁴⁵,Kékedy-Nagy László¹²³⁴,Nielsen Jesper S.¹²³⁴⁶,Campos Rui¹²³⁴,Kjems Jørgen¹²³⁴⁶,Malinowska Elżbieta⁵⁷⁸⁹¹⁰,Ferapontova Elena E.¹²³⁴

Affiliation:

1. Interdisciplinary Nanoscience Center (iNANO) and Center for DNA Nanotechnology (CDNA)

2. Aarhus University

3. DK-8000 Aarhus C

4. Denmark

5. Institute of Biotechnology

6. Department of Molecular Biology and Genetics

7. Department of Microbioanalytics

8. Faculty of Chemistry

9. Warsaw University of Technology

10. 00-664 Warsaw

Abstract

Electrochemical assay for the universal biomarker of several cancers, urokinase plasminogen activator, uPA, based on uPA-specific fluorinated RNA aptamer and methylene blue redox indicator, allows 1 pM uPA analysis in serum, making it practically useful for clinical applications.

Funder

Danish National Research Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AN/C4AN02354D

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