Direct immunoassays and their performance – theoretical modelling of the effects of antibody orientation and associated kinetics-Reference-Cited by-同舟云学术

Direct immunoassays and their performance – theoretical modelling of the effects of antibody orientation and associated kinetics

Published:2018 Issue:10 Volume:10 Page:598-604
ISSN:1757-9694
Container-title:Integrative Biology
language:en
Short-container-title:Integr. Biol.

Author:

Mackey Dana¹²³⁴^ORCID,Kelly Eilís¹²³⁴^ORCID,Nooney Robert⁵⁶⁷⁴^ORCID,O'Kennedy Richard⁸⁶⁷⁴⁹^ORCID

Affiliation:

1. School of Mathematical Sciences

2. Dublin Institute of Technology

3. Dublin 8

4. Ireland

5. Biomedical Diagnostics Institute

6. Dublin City University

7. Dublin 9

8. School of Biotechnology

9. Hamad Bin Khalifa University

Abstract

Mathematical and computational modelling are used to quantify immunoassay signals for various immobilized antibody orientations, antigen sizes and kinetic parameters, in order to determine optimal surface coverage.

Funder

Irish Research Council

Science Foundation Ireland

Publisher

Oxford University Press (OUP)

Subject

Biochemistry,Biophysics

Link

http://pubs.rsc.org/en/content/articlepdf/2018/IB/C8IB00077H

Reference18 articles.

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4. C. Dupont-Gillain , in situ , in Proteins at Interfaces III State of the art , ed. T. Horbett et al. , ACS Symposium Series, American Chemical Society, 2012

5. Significance of Antibody Orientation Unraveled: Well-Oriented Antibodies Recorded High Binding Affinity

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