A cheaper substitute for HRP: ultra-small Cu–Au bimetallic enzyme mimics with infinitesimal steric hindrance to promote catalytic lateral flow immunodetection of clenbuterol

Author:

Hu Huilan1,Tian Jiaqi1,Shu Rui1,Liu Huihui2,Wang Shaochi1,Yin Xuechi1,Wang Jianlong1ORCID,Zhang Daohong1ORCID

Affiliation:

1. College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road Yangling, 712100, Shaanxi, China

2. Shandong Key Laboratory of Marine Ecological Restoration, Shandong Marine Resource and Environment Research Institute, No. 216 Changjiang Road, Economic and Technological Development Zone, 264006, Yantai, Shandong, China

Abstract

A lateral flow immunoassay (LFIA) is developed for enzyme-catalyzed double-reading determination of clenbuterol (CLE) through the direct electrostatic adsorption of ultra-small copper–gold bimetallic enzyme mimics (USCGs) and monoclonal antibodies.

Funder

Modern Agricultural Technology Industry System of Shandong province

Publisher

Royal Society of Chemistry (RSC)

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