Achieving efficient clonal beta cells transfection using nanostraw/nanopore-assisted electroporation-Reference-Cited by-同舟云学术

Achieving efficient clonal beta cells transfection using nanostraw/nanopore-assisted electroporation

Published:2024 Issue:31 Volume:14 Page:22244-22252
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Ekstrand Frida¹,Mapar Mokhtar¹,Ruhrmann Sabrina²,Bacos Karl²,Ling Charlotte²,Prinz Christelle N.¹^ORCID

Affiliation:

1. Division of Solid State Physics, NanoLund, Lund University, 221 00 Lund, Sweden

2. Epigenetics and Diabetes Unit, Lund University Diabetes Centre, Department of Clinical Sciences, Scania University Hospital, 214 28, Malmö, Sweden

Abstract

GFP plasmids are injected in cells using nanostraw and nanopore-electroporation. After optimization, the results show that more plasmids are delivered in cells using nanostraws, but fewer cells express GFP 48 h after injection, compared to nanopores.

Funder

Stiftelsen för Strategisk Forskning

H2020 European Research Council

Novo Nordisk Fonden

Vetenskapsrådet

Bo and Kerstin Hjelt Diabetes Foundation

Region Skåne

Crafoordska Stiftelsen

NanoLund, Lunds Universitet

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/RA/D4RA02791D

Reference27 articles.

1. Lentiviral vector transduction of NOD/SCID repopulating cells results in multiple vector integrations per transduced cell: risk of insertional mutagenesis

2. Overcoming Endosomal Entrapment in Drug Delivery

3. Nanostraws for Direct Fluidic Intracellular Access

4. Nanostraw–Electroporation System for Highly Efficient Intracellular Delivery and Transfection

5. Transfection with Nanostructure Electro‐Injection is Minimally Perturbative