A CRISPR/Cas12a-powered gold/nickel foam surface-enhanced Raman spectroscopy biosensor for nucleic acid specific detection in foods

Author:

Liu Yan1ORCID,Gou Shirui2,Qiu Long3,Xu Zhiwen4,Yang Haifeng1ORCID,Yang Shiping1ORCID,Zhao Yu2

Affiliation:

1. College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, China

2. College of Life Sciences, Shanghai Normal University, Shanghai, China

3. Wuxi Tolo Biotechnology Co., Ltd, Wuxi, Jiangsu, China

4. Technology Center for Animal Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai, China

Abstract

AuNFs was used as a SERS substrate combined with CRISPR/Cas12a to achieve the specific detection of viral nucleic acids. At the same time, AuNFs could avoid the influence of Mg2+ in the CRISPR system in common SERS detection.

Publisher

Royal Society of Chemistry (RSC)

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