Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract-Reference-Cited by-同舟云学术

Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract

Published:2015 Issue:4 Volume:13 Page:1008-1012
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Ogawa Atsushi¹²³⁴,Doi Yasunori¹²³⁴

Affiliation:

1. Proteo-Science Center

2. Ehime University

3. Matsuyama

4. Japan

Abstract

We investigated the end processing and degradation of premature tRNAs in wheat germ extract (left), which led to the findings of end protectors for efficiently stabilizing an in vitro transcript (purple, right).

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/OB/C4OB02221A

Reference33 articles.

1. Cell-free expression systems for eukaryotic protein production

2. Cell-free synthetic biology: Thinking outside the cell

3. An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

4. Using a Solid-Phase Ribozyme Aminoacylation System to Reprogram the Genetic Code

5. Site-selective Post-translational Modification of Proteins Using an Unnatural Amino Acid, 3-Azidotyrosine

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