A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection-Reference-Cited by-同舟云学术

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection

Published:2023 Issue:3 Volume:148 Page:556-561
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Han Yu¹²,Hu Haihong²,Yu Lushan²,Zeng Su²,Min Jun Zhe¹^ORCID,Cai Sheng²^ORCID

Affiliation:

1. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Department of Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji 133002, Jilin Province, China

2. Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China

Abstract

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method has been developed. This strategy achieved a sensitive limit of detection and excellent selectivity for miR-146b detection.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D2AN01759H

Reference40 articles.

1. Dual Role for Argonautes in MicroRNA Processing and Posttranscriptional Regulation of MicroRNA Expression

2. MicroRNA-21 acts as an oncomir through multiple targets in human hepatocellular carcinoma

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