A simple and rapid mix-and-read assay for sensitive detection of O<sup>6</sup>-methylguanine DNA methyltransferase-Reference-Cited by-同舟云学术

A simple and rapid mix-and-read assay for sensitive detection of O⁶-methylguanine DNA methyltransferase

Published:2022 Issue:62 Volume:58 Page:8662-8665
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Liu Ming-Hao¹,Yu Wan-Tong¹,Yang Xiao-Yun²,Li Yueying³,Zhang Yan¹,Zhang Chun-Yang¹^ORCID

Affiliation:

1. A College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, China

2. Department of Pathology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China

3. Institute of Immunity and Infectious Diseases, School of Medicine and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450000, China

Abstract

We have developed a simple and rapid mix-and-read assay for the sensitive detection of O6-methylguanine DNA methyltransferase activity.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CC/D2CC03084E

Reference28 articles.

1. MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas

2. The structural basis for the mutagenicity of O6-methyl-guanine lesions

3. A novel fluorometric oligonucleotide assay to measure O 6-methylguanine DNA methyltransferase, methylpurine DNA glycosylase, 8-oxoguanine DNA glycosylase and abasic endonuclease activities: DNA repair status in human breast carcinoma cells overexpressing methylpurine DNA glycosylase

4. Regulation of DNA Alkylation Damage Repair: Lessons and Therapeutic Opportunities

5. Balancing repair and tolerance of DNA damage caused by alkylating agents

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