The role of streptavidin and its variants in catalysis by biotinylated secondary amines-Reference-Cited by-同舟云学术

The role of streptavidin and its variants in catalysis by biotinylated secondary amines

Published:2021 Issue:47 Volume:19 Page:10424-10431
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Nödling Alexander R.¹^ORCID,Santi Nicolò¹^ORCID,Castillo Raquel²,Lipka-Lloyd Magdalena¹,Jin Yi¹^ORCID,Morrill Louis C.³^ORCID,Świderek Katarzyna²^ORCID,Moliner Vicent²^ORCID,Luk Louis Y. P.¹³^ORCID

Affiliation:

1. School of Chemistry, Main Building, Cardiff University, Cardiff, CF10 3AT, UK

2. Department de Química Física i Analítica, Universitat Jaume I, Castellón, 12071, Spain

3. Cardiff Catalysis Institute, School of Chemistry, Main Building, Cardiff University, Cardiff, CF10 3AT, UK

Abstract

Here, we combine the use of host screening, protein crystallography and QM/MM molecular dynamics simulations to investigate how protein enviroment affects iminium catalysis by biotinylated secondary amines in a model 1,4 conjugate addition reaction.

Funder

Ministerio de Ciencia, Innovación y Universidades

Generalitat Valenciana

Universitat Jaume I

Leverhulme Trust

Royal Society

Wellcome

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

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