Single-cell patterning and characterisation of antibiotic persistent bacteria using bio-sCAPA-Reference-Cited by-同舟云学术

Single-cell patterning and characterisation of antibiotic persistent bacteria using bio-sCAPA

Published:2023 Issue:23 Volume:23 Page:5018-5028
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Boggon Cameron¹,Mairpady Shambat Srikanth²^ORCID,Zinkernagel Annelies S.²^ORCID,Secchi Eleonora³^ORCID,Isa Lucio¹^ORCID

Affiliation:

1. Laboratory for Soft Materials and Interfaces, Department of Materials, ETH Zürich, Switzerland

2. Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zürich, University of Zurich, Switzerland

3. Institute of Environmental Engineering, Department of Civil, Environmental, and Geomatic Engineering, ETH Zürich, Switzerland

Abstract

Bio-sCAPA allows for patterning >105 bacteria in defined geometries with single-cell precision. Antibiotic-tolerant bacteria are monitored and analysed through time, both during antibiotic exposure and during regrowth after removing the antibiotic.

Funder

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Uniscientia Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2023/LC/D3LC00611E

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5. Pareto optimality between growth-rate and lag-time couples metabolic noise to phenotypic heterogeneity in Escherichia coli

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