Sensitive detection of caspase-3 enzymatic activities and inhibitor screening by mass spectrometry with dual maleimide labelling quantitation

Author:

Ouyang Fuzhong12345,Yu Tianrong12345,Gu Chao67458,Wang Guanghui67458,Shi Rui12345,Lv Rui12345,Wu Enhui12345,Ma Chongqing12345,Guo Ruochen12345,Li Jing12345,Zaczek Anna910111213,Liu Jian12345ORCID

Affiliation:

1. Institute of Functional Nano and Soft Materials (FUNSOM)

2. Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices

3. Joint International Research Laboratory of Carbon-Based Materials and Devices

4. Soochow University

5. Suzhou

6. Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases

7. College of Pharmaceutical Sciences

8. China

9. Medical Biotechnology Department

10. Intercollegiate Faculty of Biotechnology

11. University of Gdansk and Medical University of Gdansk

12. Gdansk

13. Poland

Abstract

There is a great need to develop sensitive and specific methods for quantitative analysis of caspase-3 activities in cell apoptosis.

Funder

National Basic Research Program of China

National Natural Science Foundation of China

Collaborative Innovation Center of Suzhou Nano Science and Technology

Priority Academic Program Development of Jiangsu Higher Education Institutions

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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