A point-of-care microfluidic biosensing system for rapid and ultrasensitive nucleic acid detection from clinical samples

Author:

Zhang Yuxuan12ORCID,Song Yang12,Weng Zhengyan12,Yang Jie3ORCID,Avery Lori4,Dieckhaus Kevin D.5,Lai Rebecca Y.6,Gao Xue378,Zhang Yi12ORCID

Affiliation:

1. Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA

2. Institute of Materials Science, University of Connecticut, Storrs, CT 06269, USA

3. Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA

4. Department of Pathology and Laboratory Medicine, UConn Health, Farmington, CT 06030, USA

5. Division of Infectious Diseases, Department of Medicine, UConn Health, Farmington, CT 06030, USA

6. Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA

7. Department of Bioengineering, Rice University, Houston, TX 77005, USA

8. Department of Chemistry, Rice University, Houston, TX 77005, USA

Abstract

A point-of-care microfluidic device takes unprocessed clinical samples, actively lyses viral RNAs, and performs the target amplification-free nucleic acid detection with a limit of detection down to 10 aM with 25 min sample-to-result time.

Funder

University of Connecticut

Division of Electrical, Communications and Cyber Systems

Rice University

Division of Chemical, Bioengineering, Environmental, and Transport Systems

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Reference54 articles.

1. Diagnostics for SARS-CoV-2 infections

2. World Health Organization , 2020 , https://www.who.int/news-room/commentaries/detail/advice-on-the-use-of-point-of-care-immunodiagnostic-tests-for-covid-19

3. Rapid point-of-care testing for SARS-CoV-2 in a community screening setting shows low sensitivity

4. Diagnosing COVID-19: The Disease and Tools for Detection

5. Antibody responses to SARS-CoV-2 in patients with COVID-19

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